Plasmon heating for localized desorption of cells immobilized on a biochip

Plasmon heating for localized desorption of cells immobilized on a biochip

Élodie Engel Radoslaw Bombera Loïc Leroy Roberto Calemczuk Loïc Laplatine Dieudonné R. Baganizi Patrice N. Marche Yoann Roupioz Thierry Livache 

Univ. Grenoble Alpes, INAC-SPrAM, 38000 Grenoble, France

CNRS, SPrAM, 38000 Grenoble, France

CEA, INAC-SPrAM, 38000 Grenoble, France

Institut Albert Bonniot INSERM-UJF U823, 38000 Grenoble, France

Corresponding Author Email:
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This work proposes a miniaturized system able to perform multiple cell capture followed by cell-type selective release from a gold-coated biochip surface. Unlabeled lymphocytes are first specifically captured onto a DNA array by antibody-DNA conjugates. Then a laser illuminates, from below the sample under total reflection conditions, the cells spots to be released. In well-defined conditions, the energy of incoming photons is absorbed by surface electron oscillations, and then heat converted and dissipated into the neighboring medium. Because of the temperature rising, the double-stranded DNA dehybridate which enables a spatially and temporaly controlled liberation of corresponding biological objects (i.e. lymphocytes) upon laser-induced local heating.


surface plasmon resonance, DNA biochip, cell sorting.

1. Introduction
2. Principe de l’expérience
3. Matériel et méthodes
4. Résultats et discussion
5. Conclusion

Les auteurs remercient l’Agence Nationale pour la Recherche pour le financement de ce projet (Multicells, ANR-BLANC-1519-01) ainsi que la fondation Nanosciences RTRA pour le financement de la thèse de R.B. Ce travail a également été soutenu par le Labex ARCANE (ANR-11-LABX-0003-01).


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