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This work proposes a miniaturized system able to perform multiple cell capture followed by cell-type selective release from a gold-coated biochip surface. Unlabeled lymphocytes are first specifically captured onto a DNA array by antibody-DNA conjugates. Then a laser illuminates, from below the sample under total reflection conditions, the cells spots to be released. In well-defined conditions, the energy of incoming photons is absorbed by surface electron oscillations, and then heat converted and dissipated into the neighboring medium. Because of the temperature rising, the double-stranded DNA dehybridate which enables a spatially and temporaly controlled liberation of corresponding biological objects (i.e. lymphocytes) upon laser-induced local heating.
surface plasmon resonance, DNA biochip, cell sorting.
Les auteurs remercient l’Agence Nationale pour la Recherche pour le financement de ce projet (Multicells, ANR-BLANC-1519-01) ainsi que la fondation Nanosciences RTRA pour le financement de la thèse de R.B. Ce travail a également été soutenu par le Labex ARCANE (ANR-11-LABX-0003-01).
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