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Electrochemical Study of a Nano Vesicular Artificial Peroxidase on a Functional Nano Complex Modified Glassy Carbon Electrode

School of Life Sciences, Henan University, Kaifeng 475000, China

Institute of Biochemistry and Biophysics, University of Tehran, Tehran, Iran

Chemistry & Chemical Engineering Research Center of Iran (CCERCI), Tehran, Iran

Corresponding Author Email:

hongjun@henu.edu.cn

Received:

27 December 2012

| |

Accepted:

21 February 2013

| | Citation

v16n02a05_p089-095.pdf

OPEN ACCESS

Abstract:

A nano vesicular was constructed with the complex consisting of hemin, imidazole Gemini and sodium dodecyl sulfate (SDS) in 50 mM phosphate buffer solution (PBS). The novel nano structure acted as an efficient artificial peroxidase (AP). The AP was also immobi- lized on a carboxyl functionalized multi-walled carbon nano-tubes (MWCNTs-COOH) and gold nano-particles (AuNPs) nano-complex modified glassy carbon (GC) electrode, with a formal potential of -31± 2 mV vs. Ag/AgCl in 50 mM PBS, at a scan rate of 0.05 Vs^-1. The heterogeneous electron transfer constant (ks) was evaluated to be 5.4 ± 0.1s^-1. The modified electrode can be used to detect hydrogen per- oxide in the range from 0.03 to 160 μM linearly, with a detection limit of 0.03μM. The apparent Michaelis-Menten constant (K_m^app) of AP modified electrode was evaluated to be 0.034 ± 0.003mM.

Keywords:

Nano Vesicular; Artificial peroxidase; functional nano complex; hydrogen peroxide; Biosensor

1. Introduction

2. Experimental

3. Results and Discussion

4. Conclusions

5. Acknowledgements

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Electrochemical Study of a Nano Vesicular Artificial Peroxidase on a Functional Nano Complex Modified Glassy Carbon Electrode