Experiment and mechanism study on microbial improvement of dredger fill

Experiment and mechanism study on microbial improvement of dredger fill

Xiaoduo Ou  Xin Pan  Kaiwen Hou  Xiantai Yin 

College of Civil Engineering and Architecture, Guangxi University, Nanning 530004, China

Key Laboratory of Disaster Prevention and Structural Safety of Ministry of Education, Guangxi University, Nanning 530004, China

Guangxi Metal Tailings Safety Prevention and Control Engineering Technology Research Center, Nanning 530004, China

Nanning Wangting Project Mangement Co.,Ltd, Nanning 530000, China

Corresponding Author Email: 
ouxiaoduo@163.com
Page: 
189-208
|
DOI: 
https://doi.org/10.3166/ACSM.41.189-208
Received: 
| |
Accepted: 
| | Citation

OPEN ACCESS

Abstract: 

Considering the soil improvement effect of micro-organic metabolites, Aspergillus niger, Agrobacterium radiobacter and Bacillus licheniformis were selected for experiments on the microbial improvement of hydraulic fill soil. Specifically, different microbial strains and culture media were added to hydraulic fill soil, and subjected to triaxial shear tests. Then, the specific surface area (SSA), chemical composition and mineral composition of the microorganism-modified soil were measured and analysed. The results show that, the soil samples mixed with all microbial strains, except Aspergillus niger, underwent the reduction of the peak deviator stress, the increase of cohensive force and the decrease of the internal friction angle. The soil samples had similar mineral composition. After the introduction of microbial strains and culture media, the valent cation content and the friction between soil grains of all samples both dropped, leading to a decline in the internal friction angle. The SSA of soil grains added with Agrobacterium radiobacter increased by 49%, while that of soil grains added with Bacillus licheniformis grew by 45%. This is because the strains altered the connection state between soil grains and the soil structure, which enlarged the soil particles and enhanced the cohesive force. The research findings reveal the good effect of microbial technology in the improvement of hydraulic fill soil, and lays a soild basis for the application of the microbial improvement technology

Keywords: 

microbial improvement, hydraulic fill, triaxial shear test, osmotic coefficient

1. Introduction
2. Selection of microbial strains and the properties of soil samples
3. Test preparation and method
4. Analysis of test results
5. Study on the mechanism of microbial improvement
6. Conclusion
Acknowledgements

Project funded by National Natural Science Foundation (51768006)

  References

Chou C. W., Eric S., Asce A. M., Ahmet H. A. (2011). Biocalcification of sand through ureolysis. Journal of Geotechnical and Geoenvironmental Engineering, ASCE, Vol. 137, No. 12, pp. 1179-1189. https://doi.org/10.1061/(ASCE)GT.1943-5606.0000532

Chu J., Ivanov V., He J., Naeimi M., Li B. (2011). Development of Microbial Geotechnology in Singapore. Geotechnical Special Publication, No. 211, pp. 4070-4078.

Chu J., Stabnikov V., Ivanov V. (2012). Microbially induced calcium carbonate precipitation on surface or in the bulk of soil. Geomicrobiology Journal, No. 29, pp. 544-549. https://doi.org/10.1080/01490451.2011.592929

DeJong J. T., Mortensenb B. M., Martinez B. C., Nelson D. (2010). Biomediated soil improvement. Ecological Engineering, No. 36, pp. 197-210. https://doi.org/10.1016/j.ecoleng.2008.12.029

DeJong J. T., Soga K., Banwart S. A., Whalley W., Ginn T., Nelson D., Mortensen B., Martinez B., Barkouki T. (2011). Soil engineering in vivo: Harnessing natural biogeochemical systems for sustainable, multi-functional engineering solutions. Journal of the Royal Society, Interface, No. 8, pp. 1-15. https://doi.org/10.1098/rsif.2010.0270

DeJong J. T., Soga K., Kavazanjian E., Burns S., Van Paassen L. A., Al Qabany A., Aydilek A., Bang S. S., Burbank M. (2013). Biogeo-chemical processes and geotechnical applications: progress, opportunities and challenges. Geotechnique, No. 63, pp. 287-301.

Dick J., De Windt W., De Graef B., Saveyn H., Van Der Meeren P., De Belie N., Verstraete W. (2006). Bio-deposition of a calcium carbonate layer on degraded limestone by Bacillus species. Biodegradation, Vol. 17, No. 4, pp. 357-367. https://doi.org/10.1007/s10532-005-9006-x

Hammes F., Boon N., De Villiers J., Verstraete W., Siciliano S. (2003). Strain-specific ureolytic microbial calcium carbonate precipitation. Applied & Environmental Microbiology, Vol. 69, No. 8, pp. 4901-4909. https://doi.org/10.1128/AEM.69.8.4901-4909.2003

Kitamura M., Konno H., Yasui A., Masuoka H. (2002). Controlling factors and mechanism of reactive crystallization of calcium carbonte polymorphs from calcium hydroxide suspensions. Journal of Crystal Growth, Vol. 236, No. 1-3, pp. 323-332. https://doi.org/10.1016/S0022-0248(01)02082-6

Marinari S., Masciandaro G., Ceccanti B., Grego S. (2000). Influence of organic and mineral fertilizers on soil biological and physical properties. Bioresesource Technology, Vol. 72, No. 1, pp. 9-17. https://doi.org/10.1016/s0960-8524(99)00094-2

Namkoong W., Hwang E. Y., Park J. S., Choi J. K. (2002). Bioremediation of diesel-contaminated soil with composting. Environmental Pollution, Vol. 119, No. 1, pp. 23-31. https://doi.org/10.1016/S0269-7491(01)00328-1

Van Paassen L. A., Daza C. M., Staal M., Sorokin Dvan der Zon Wvan Loosdrecht M (2010). Potential soil reinforcement by biological denitrification. Ecological Engineering, Vol. 36, No. 2, pp. 168-175. https://doi.org/10.1016/j.ecoleng.2009.03.026

Yang P., Tang Y. Q., Wang J. X., Yang Y. (2012). Test on consolidation of dredger fill by cube grid of plastic drain board preinstalled. Engineering Geology, Vol. 127, pp. 81-85. https://doi.org/10.1016/j.enggeo.2012.01.004